tag:blogger.com,1999:blog-7229885.post1634432906287392853..comments2024-03-27T01:58:22.445-07:00Comments on The Gazetteer: The Advent Jukebox, Day 20...Rudolph's Red Fluorescent Nose.RossKhttp://www.blogger.com/profile/07677239332112652522noreply@blogger.comBlogger8125tag:blogger.com,1999:blog-7229885.post-66502792084677221712017-12-21T15:38:43.056-08:002017-12-21T15:38:43.056-08:00You're bang on Glen.....Shimomura isolated the...You're bang on Glen.....Shimomura isolated the protein only (i.e. he didn't clone the gene) from all that jellyfish tissue. Thus, despite the fact that Shimomura figured out how the protein worked (i.e. UV light excited it so that it emitted the fluorescent light) there was no way to easily bang said protein into cells. Instead you needed to sequence/clone the gene that encoded the protein so that it could be 'expressed' (i.e. go from DNA through RNA to protein) within cells...That required somebody to clone the gene by going backward from a wee bit of the protein's sequence (i.e. go from protein back to DNA)...That's what Prasher did which is why everybody involved in the 'prize' recognized the importance of his contribution.<br /><br /><br />.<br /><br />RossKhttps://www.blogger.com/profile/07677239332112652522noreply@blogger.comtag:blogger.com,1999:blog-7229885.post-49939861748135727892017-12-21T15:02:27.896-08:002017-12-21T15:02:27.896-08:00That is an incredible (and sad) story on Prasher! ...That is an incredible (and sad) story on Prasher! Thanks for the link. Seems downright crazy!Glen Clarkhttps://www.blogger.com/profile/10225195536266477648noreply@blogger.comtag:blogger.com,1999:blog-7229885.post-49270322504800037222017-12-21T14:59:11.868-08:002017-12-21T14:59:11.868-08:00Interesting stuff!
I'm out of my league here,...Interesting stuff! <br />I'm out of my league here, but I thought it wasn't until the 80's that they could use GFP as a tracer.After that the use exploded.Glen Clarkhttps://www.blogger.com/profile/10225195536266477648noreply@blogger.comtag:blogger.com,1999:blog-7229885.post-17507038899896431392017-12-21T12:02:26.773-08:002017-12-21T12:02:26.773-08:00Thanks Hugh!
.Thanks Hugh!<br /><br />.RossKhttps://www.blogger.com/profile/07677239332112652522noreply@blogger.comtag:blogger.com,1999:blog-7229885.post-87439627414089213162017-12-21T12:01:53.230-08:002017-12-21T12:01:53.230-08:00Glen--
This particular three way-shared Nobel pri...Glen--<br /><br />This particular three way-shared Nobel prize is really interesting that way...Simomura was doing pure 'basic science'...Martie Chalfie moved it along by applying it when he showed that he could tag the green fluorescent protein (GFP) on to other proteins and then stick this chimera into any cell type he wanted, including cells in developing round worms. This made it possible to track the location and movement of just about any protein in any cell....Then, Roger Tsien really did the application work and reverse-engineered the beejebuz out of the GFP gene (by site-directed mutation using, originally a strategy developed by local Nobel wunderkind Michael Smith) to make a whole host of differently coloured fluorescent proteins which meant that you could tag multiple proteins in the same cell. The upshot, this came full circle back to pure basic science so that folks could 'live image' just about anything protein they wanted and see how those proteins interact, move and/or function in real time under the microscope.<br /><br />There was one scientist who wrote a really important chapter in this story who got lost in the shuffle and did not share in the prize. That was a young guy named Douglas Prasher who first cloned a serviceable version of the GFP gene that Chalfie and Tsien subsequently used. Unfortunately Prasher lost his 'basic science' funding and gave the clone away to all kinds of labs including Chalfie's who he worked with on 'expressing' it a bit. Chalfie was very magnanimous about this and said the following when the Nobel was awarded:<br /><br /><i>..."(Douglas Prasher's) work was critical and essential for the work we did in our lab. They could've easily given the prize to Douglas and the other two and left me out."...</i><br /><br />Prasher's life story is <a href="https://web.archive.org/web/20081011230049/http://www.capecodonline.com/apps/pbcs.dll/article?AID=%2F20081011%2FNEWS%2F810110328" rel="nofollow">an amazing one.</a><br /><br />_______<br /><br />Thanks Lew!<br /><br />Not sure it is quite as sharp as yours however.<br /><br />Also, I have come to the conclusion that my memory has become crammed with too much stuff which sometimes slows instantaneous retrieval, so much so that Bigger E now creams me at Jeopardy, episodes of which my Dad/her Granddad saves on his PVR so that we can watch them by the bushels full over the holidays.<br /><br />Truth be told E., would probably also <a href="https://www.youtube.com/watch?v=3wXkItoqHew" rel="nofollow">cream the Kevin Bacon character in Barry Levinson's 'Diner'...</a><br /><br />Weirdly, my Dad kind of looks like Alex Trebek whom he detests due to his fauxian superiority complex and the smarminess he displays towards the contestants and their stories told after the first commercial break...It's a crazy, mixed-up world we live in.<br /><br /><br />.RossKhttps://www.blogger.com/profile/07677239332112652522noreply@blogger.comtag:blogger.com,1999:blog-7229885.post-76941067478001570682017-12-21T11:48:30.427-08:002017-12-21T11:48:30.427-08:00http://vancouversun.com/pmn/business-pmn/aecon-par...http://vancouversun.com/pmn/business-pmn/aecon-partnership-named-preferred-proponent-for-site-c-generating-station/wcm/1cfa8cb8-2841-4f12-8739-46cf1b2215d9<br /><br />Article leaves something out:<br /><br />https://www.theglobeandmail.com/report-on-business/aecon-shareholders-approve-15-billion-takeover-offer-by-chinese-company/article37381990/Hughnoreply@blogger.comtag:blogger.com,1999:blog-7229885.post-42033098133386829342017-12-21T08:14:48.747-08:002017-12-21T08:14:48.747-08:00RossK, you've written more than once that you&...RossK, you've written more than once that you've got a memory and you plan to use it.<br /><br />I'm glad of that.Lewnoreply@blogger.comtag:blogger.com,1999:blog-7229885.post-43157820406863398112017-12-21T06:46:24.649-08:002017-12-21T06:46:24.649-08:00Thanks for this. Both the lovely story, song and r...Thanks for this. Both the lovely story, song and reference to Osamu Shimomura. Fascinating story that reminds me of an article by Stephen Jay Gould that revisited the debate of "basic science" vs. "applied science". Glen Clarkhttps://www.blogger.com/profile/10225195536266477648noreply@blogger.com