
BaconNumbersInTheCellular
MachineVilleTruth be told, it turns out that I'm a bit of a scientific
Zelig who has been lucky enough to be in the right place at the right time a number of times for reasons I can't really explain, at least not in purely scientific terms.
For example, when I was a post-doctoral fellow, which is the final stage in the long apprenticeship of a junior science geek, there was this really sharp young post-doc named Goberdhan working in the lab next door who needed some human cells with a limited lifespan because he wanted to subject them to a test he was developing that would turn cells blue when they were old, worn-out and ready to stop dividing.
Now, back then, in the early '90s, keeping human cells going/dividing that hadn't become 'immortal', either because they were derived from tumors, like, say,
the infamous cells isolated from Henrieta Lacks' cervical cancer, or because they had been infected with viruses that turned off important cell cycle checkpoints so that they could get past the then still mysterious
'Hayflick limit', wasn't an easy task.
And here's where the luck of the Zelig comes in...
Before I was a post-doc, I had been lucky enough to do my PhD with
Nelly Auersperg who was a world expert in culturing human cells from normal tissues with just the type of limited lifespan that Goberdhan was looking for. So, I got Nelly to ship me some of her cells and made like that
old Trooper song in the tissue culture room. Essentially, I grew some of Nelly's cells for a good/short time (i.e. so that they were still happily dividing) and some of them for a long time (i.e. so that they were starting to get tired just before they stopped dividing for good). Then I handed the cells over to Goberdhan so that he could try his little trick. Lo-and-behold, it was the cells that were old and tuckered out that turned blue.
Why does this matter?
Well, from Zelig perspective #1, I was lucky enough to become a co-author on
Goberdhan's paper which has gone on to become a block-buster in science-geek terms with over 8,000 citations (that's a lot - I'm very happy that I have a number of other papers with hundreds of citations, but none of those even comes close to that 8,000 plus number - and fellow geeks and university administrators pay attention to those kinds of stats, kind of like baseball players and team owners with
OPS+, if you get my drift).
As for Zelig perspective #2, it turns out that that Goberdhan's test, which he developed while working for one of the smartest scientists I've ever met,
Judy Campisi, ushered in the modern version of the field of
'senescence' which, for good reason, is now all the rage in anti-aging circles.
All this happened more than thirty years ago while I was working for another super smart scientist named
Mina Bissell at the Lawrence Berkeley National Laboratory
which is perched on the hill up above the UC Berkeley campus.Another nearby lab was run by yet another unbelievably sharp scientist named
Shyamala Gopalan, shown at the top of the post, who was at that time working to figure out the functional relationships
between estrogen receptors, progesterone receptors and a funky cell surface protein called Her2. Anyone who has dealt with, or has a loved one who has dealt with, breast cancer will know those terms because they are three of the most important biomarkers of the disease that can now be targeted using rational therapeutics rather than the old fashioned slash-and-burn therapeutics that still need to be used when they are not present.
I never collaborated or worked directly with Shyamala, who was tough as nails and did not suffer fools gladly. However, I knew a little bit about steroid biochemistry due to my
previous work with adrenocortical cells which she appreciated given that estrogen and progesterone are both steroids. I think she also gave me a bit of a benefit of the doubt because I was Canadian and had spent a little time as a gradual student at the Lady Davis Institute in Montreal where she had been before Mina helped bring her back to Berkeley.
Which brings me to Zelig perspective #3, which has nothing whatsoever to do with science, because...
If you haven't figured it out already, it turns out that
Shyamala was Kamala Harris' mom.Imagine that!
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Why was Goberdhan's paper a blockbuster back in the days when video stores were king?...Because you can do his little trick in situ (i.e. in animal and human tissue and biopsies)...Thus, this became the first, and still only really good, way to track aging cells in the body in all sorts of situations, including during disease manifestations.
Dr. Gopalan never mentioned her kids when we chatted way back when... After all, her kids were all grown up by then (Kamala graduated from law school in 1989)...But she never missed a chance to ask how the then very tiny (now Bigger) e. was doing...Gosh...As I've said around here more than once before, it really does all go by in a flash...
.